B.S., 1980, Purdue University, West Lafayette, IN
M.S., 1982, University of Wisconsin-Madison
Ph.D., 1984, University of Wisconsin-Madison
Postdoctoral Fellow, University of Wisconsin Medical School and University of Virginia Medical School
Muscle cells are unique in their ability to generate large amounts of force in a short period of time. In skeletal, cardiac and smooth muscle cells the method by which force is generated, the time scale required, and the mechanism for regulation are similar but yet unique. The differences within and between these tissues allow them to maximally perform their required functions.
One of the many differences between the muscle types are the contractile proteins themselves--the proteins directly involved in force generation. The major contractile proteins, actin and myosin, show tissue-specific types. These isoforms allow optimization of each muscle type for its specific function, including variation in speed of shortening, amount of force generated, and energy consumption. Differences in numerous other proteins within these cells, as well as their anatomical organization, regulatory mechanisms, innervation, etc., all contribute to the diversity between these tissues.
Research work in this lab involves the study of contractile regulatory and cytoskeletal proteins in muscle including their expression, regulation and function in SM contraction. Molecular, mechanical, biochemical, histochemical, and immunological approaches are used to further our understanding of the function and regulation of these proteins and their isoforms.
Huang, Q., Babu, G.J., Periasamy, M., and Eddinger T.J. 2013. SMB myosin heavy chain knockout enhances tonic contraction and reduces the rate of force generation in ileum and stomach antrum. Am. J. Physiol. Cell Physiol. 304(2): C194-206.
Menon, A., Eddinger, T.J., Wang, H., Wendell, D.C., Toth, J.M., and LaDisa, J.F. 2012. Altered hemodynamics, endothelial function and protein expression occur with aortic coarctation and persist after repair. Am J Physiol Heart Circ Physiol. 303: H1304-H1318.
Menon, A., Wendell, D.C., Wang, H., Eddinger, T.J., Toth, J.M., Dholakia, R.J., Larsen, P.M., Jensen, E.S., and LaDisa, J.F. Jr. 2012. A coupled experimental and computational approach to quantify deleterious hemodynamics, vascular alterations, and mechanisms of long-term morbidity in response to aortic coarctation. J Pharmacol Toxicol Methods. 65(1):18-28. Epub. Nov 4, 2011.
Eddinger, T.J. 2009. Unique contractile and structural protein expression in dog ileal inner circular smooth muscle. J. Smooth Muscle Research, 45: 217-230.
Govindaraju, S.R., J.L. Bain, T.J. Eddinger, D.A. Riley. 2008. Vibration causes acute vascular injury in a two-step process: vasoconstriction and vacuole disruption. Anat. Rec. (Hoboken). 291: 999-1006.
Eddinger, T.J., D.P. Meer, A.S. Miner, J.T. Meehl, A.S. Rovner, and P.H. Ratz. 2007. Potent inhibition of Arterial smooth muscle tonic contractions by the selective myosin II inhibitor, Blebbistatin, J. Pharmacol. Exp. Ther. 2007 320: 865-870.
Eddinger, T.J. and D.P. Meer. 2007. Myosin II Isoforms in Smooth Muscle: Heterogeneity and Function. Am J Physiol Cell Physiol., 293: C493-C508.
Rondelli CM, Szasz IT, Kayal A, Thakali K, Watson RE, Rowner AS, Eddinger TJ, Fink GD, and Watts SW. 2007. Preferential Myosin Heavy Chain Isoform B Expression May Contribute to the Faster Velocity of Contraction in Veins Versus Arteries. J Vasc Res 44: 264-272.
Eddinger, T.J., J.D. Schiebout and D.R. Swartz. 2007. Adherens junction associated protein distribution differs in smooth muscle tissue and acutely isolated cells. Am. J. Phys. Gastro. Liver Physiol., 292: G684-G697.
Yu Zhang and T.J. Eddinger. PKCα and CPI-17 expression and spatial-temporal distribution with activation in pig stomach antrum and fundus. Experimental Biology, April 9-13, 2011. Washington DC.
Qian Huang and T.J. Eddinger. Smooth muscle phasic vs. tonic contractions are regulated by different myosin heavy chain isoforms. Experimental Biology, April 9-13, 2011. Washington DC.
National Academies Education Fellow in Life Sciences (2007)
Dr. Eddinger is currently accepting new Ph.D. students into his lab